## What 5-Amino-1MQ is
5-Amino-1MQ is a selective, membrane-permeable small-molecule NNMT inhibitor (EC50 ~2.3 µM in adipocytes) that blocks nicotinamide methylation to 1-MNA, preserving nicotinamide for NAD+ salvage, elevating NAD+ 1.2-1.6 fold, and activating Sirt1 for metabolic benefits including ~35% adipose reduction without appetite effects. It has a molecular formula C10H11N2O, mass 173.21 Da. Common synonyms in the literature include 5-Amino-1-Methylquinolinium, NNMT inhibitor, and 5A1MQ.
## What it's studied for
Investigation of 5-Amino-1MQ to date is primarily preclinical, with the majority of citations concentrated in animal models and in-vitro assays rather than randomized controlled human trials. Published work referencing 5-Amino-1MQ spans Adipocyte studies (in vitro): Differentiated adipocytes treated with 5-Amino-1MQ showed concentration-dependent NNMT inhibition with EC50 ~2.3 µM. Treatment reduced intracellular 1-MNA, elevated NAD+ 1.2-1.6 fold at 1-60 µM doses, increased SAM levels, and suppressed lipogenesis markers, Diet-induced obesity model (DIO mice): Systemic 5-Amino-1MQ treatment produced ~5.1% body weight loss (2.0 ± 0.6 g) and ~35% reduction in epididymal white adipose tissue (EWAT) mass. Adipocytes were smaller with reduced lipid content. Plasma cholesterol decreased. Critically, food intake was unchanged—effects were metabolic, not appetite-mediated, and Muscle and exercise studies: Aged mice treated with 5-Amino-1MQ showed improved running distance and reduced exercise recovery time, likely mediated by NAD+ elevation supporting mitochondrial function and Sirt1 activity in muscle tissue. Proposed mechanisms in the literature include Competitive NNMT inhibition: binds catalytic pocket, blocks nicotinamide → 1-MNA conversion (EC50 ~2.3 µM) and NAD+ salvage preservation: nicotinamide enters NAMPT → NMN → NAD+ pathway instead of methylation. None of this material describes therapeutic outcomes in humans, and BioInfinity does not sell, market, or provide dosing guidance for 5-Amino-1MQ outside laboratory research.
## Laboratory handling — storage and reconstitution
Lyophilized 5-Amino-1MQ arrives as a fluffy off-white solid inside a sealed sterile vial. Because it ships as a small-molecule research compound, the powder is best stored between 2–8 °C prior to reconstitution and returned to −20 °C in aliquots for long-term storage post-reconstitution. Reconstitution is typically performed with bacteriostatic water (0.9 % benzyl alcohol preserved) rather than sterile water because the preservative extends solution stability once the vial has been punctured. Warming the reconstituted solution to room temperature before drawing helps redissolve any peptide that has settled against the vial wall. Avoid repeated freeze–thaw cycles: for peptides in this molecular-weight range (173.21 Da), freeze–thaw stress is one of the most common causes of loss-of-purity between batches.
## How this batch is verified
Every batch of 5-Amino-1MQ ships with a batch-specific Certificate of Analysis produced by an ISO-accredited third-party analytical laboratory. Reversed-phase HPLC confirms the assay against the expected 173.21 Da mass (C10H11N2O); mass spectrometry validates the exact monoisotopic mass; endotoxin (LAL) testing confirms bacterial-contamination limits appropriate for laboratory research; and residual-solvent panels confirm the manufacturing pipeline. The lot number on the outer label matches the COA on the public /coa-library page — every purity claim is independently verifiable before you open the vial.
## Related research
For the full mechanistic profile, published references, and constraint notes on 5-Amino-1MQ, see the [5-Amino-1MQ research page](/research/5-amino-1mq). All references cited on that page are peer-reviewed primary literature.
**Research use only.** 5-Amino-1MQ is sold strictly for in-vitro and animal research. It is not a drug, food, supplement, or cosmetic and is not intended for human or veterinary consumption. No statements on this page describe or imply any therapeutic effect.
Researchers should refer to peer-reviewed publications for primary sources on this compound. 5-Amino-1MQ is sold strictly for laboratory research use and is not intended for human or veterinary use.
